Peroxidase chromogen-substrate 3-3’ diaminobezidine (DAB) reagent is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives chocolate brown reddish color and is compatible with hematoxylin, nuclear fast red, methylene blue or Methyl green counterstain. This chromogen with above counterstains are insoluble in alcohol and organic solvents (dehydrating agents); therefore can be mounted with organic mounting mediums; e.g. our Organo mounting medium or others. DAB can also be mounted with aqueous mounting medium if hematoxylin is used as counter stain.
Reagent B: concentrated Buffer 5 ml (yellow color cap)
Reagent C: DAB chromogen tablets 20 (amber color cap)
Reagent S: substrate, H2O2 3 ml (white color cap)