GeneTex
United States (US)

DACH1 antibody, Internal

GTX88629_2128_WB_18121411_785.jpg

Cat No. GTX88629

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88629-PEP).

Specificity/Sensitivity

This antibody is expected to recognise all three reported isoforms(NP_542937.1; NP_542938.1; NP_004383.2).

Predict Reactivity

Mouse, Rat, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence SPVENTPQNNECK, from the internal region of the protein sequence according to NP_542937.1; NP_542938.1; NP_004383.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dachshund Family Transcription Factor 1,Dach,Dach1

Background

This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES
GTX88629_2128_WB_18121411_785.jpg

GTX88629 WB Image

WB analysis of human kidney lysate using GTX88629 DACH1 antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer