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DACT3 antibody [2A5]

Anti-DACT3 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX50032
Anti-DACT3 antibody [2A5] used in Western Blot (WB). GTX50032

Cat No. GTX50032

Host

Mouse

Clonality

Monoclonal

Clone Name

2A5

Isotype

IgG1

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:250 ~ 500.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human DACT3 (89-202aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dishevelled Binding Antagonist Of Beta Catenin 3 , Dapper3 , Rrr1 , Dact3

Background

DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications.

Database

Research Area

DATA IMAGES

Anti-DACT3 antibody [2A5] used in IHC (Paraffin sections) (IHC-P). GTX50032

GTX50032 IHC-P Image

Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human DACT3 (1:50, GTX50032) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

Anti-DACT3 antibody [2A5] used in Western Blot (WB). GTX50032

GTX50032 WB Image

Cell lysates of mouse brain and 293T (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human DACT3 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf