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DAD1 antibody

Anti-DAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85083
Anti-DAD1 antibody used in Western Blot (WB). GTX85083
Anti-DAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85083

Cat. No. GTX85083

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all DAD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

Hep G2 Cell Slide(GTX25530) , HepG2 Cell Lysate(GTX27900)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.The immunogen is located within the last 50 amino acids of DAD1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

defender against cell death 1 , OST2

Background

Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death (1), a process important for normal organism development and tissue homeostasis (2). DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity (3,4). Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death (5,6). Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas (7), indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.

Database

Research Area

DATA IMAGES

Anti-DAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85083

GTX85083 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX85083 DAD1 antibody.
Working concentration : 10 μg/ml

Anti-DAD1 antibody used in Western Blot (WB). GTX85083

GTX85083 WB Image

WB analysis of HepG2 cell lysate using GTX85083 DAD1 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-DAD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX85083

GTX85083 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX85083 DAD1 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399