GeneTex
United States (US)

DAG1 antibody, Internal

GTX88089_1088_WB_18121411_345.jpg
GTX88089_1087_WB_18121411_139.jpg

Cat No. GTX88089

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88089-PEP).

Specificity/Sensitivity

This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-HVGKHEYFMHATDK, from the internal region of the protein sequence according to NP_004384.4.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dystroglycan 1,156Dag,A3A,Agrnr,Dag,Mddga9,Mddgc7,Mddgc9,Dag1

Cellular Localization

The monomeric form translocates to the nucleus via the action of importins and depends on RAN. Nuclear transport is inhibited by Tyr-892 phosphorylation. In skeletal muscle, this phosphorylated form locates to a vesicular internal membrane compartment. In

Background

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010]

Database

Research Area

DATA IMAGES
GTX88089_1088_WB_18121411_345.jpg

GTX88089 WB Image

WB analysis of human skeletal muscle lysate using GTX88089 DAG1 antibody, Internal.
Dilution : 0.2µg/ml
Loading : 35µg protein in RIPA buffer

GTX88089_1087_WB_18121411_139.jpg

GTX88089 WB Image

WB analysis of mouse and rat skeletal muscle lysate using GTX88089 DAG1 antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329