APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88089-PEP).
Product Note
This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-HVGKHEYFMHATDK, from the internal region of the protein sequence according to NP_004384.4.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dystroglycan 1 , 156DAG , A3a , AGRNR , DAG , LGMDR16 , MDDGA9 , MDDGC7 , MDDGC9
Cellular Localization
Cytoplasm, cytoskeleton , nucleoplasm
Background
This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
|
GTX88089 WB Image
WB analysis of human skeletal muscle lysate using GTX88089 DAG1 antibody, Internal.
Dilution : 0.2μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88089 WB Image
WB analysis of mouse and rat skeletal muscle lysate using GTX88089 DAG1 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
|
REFERENCE
There are currently no references for DAG1 antibody, Internal (GTX88089). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DAG1 antibody, Internal (GTX88089). Be the first to share your experience with this product.
