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DAG1 antibody

Anti-DAG1 antibody used in Western Blot (WB). GTX105038
Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105038
Anti-DAG1 antibody used in Western Blot (WB). GTX105038
Anti-DAG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105038
Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105038
Anti-DAG1 antibody used in Western Blot (WB). GTX105038
Anti-DAG1 antibody used in Western Blot (WB). GTX105038

Cat No. GTX105038

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat, Horse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

U87-MG , U87-MG membrane fraction extract

Predict Reactivity

Rabbit, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of Human DAG1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dystroglycan 1 , 156DAG , A3a , AGRNR , DAG , LGMDR16 , MDDGA9 , MDDGC7 , MDDGC9

Cellular Localization

Alpha-dystroglycan: Secreted , extracellular space , Beta-dystroglycan: Cell membrane , Cytoplasm , cytoskeleton

Background

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DAG1 antibody used in Western Blot (WB). GTX105038

GTX105038 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody (GTX105038) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105038

GTX105038 ICC/IF Image

alpha Dystroglycan antibody detects alpha Dystroglycan protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Dystroglycan protein stained by alpha Dystroglycan antibody (GTX105038) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-DAG1 antibody used in Western Blot (WB). GTX105038

GTX105038 WB Image

DAG1 antibody detects DAG1 protein by western blot analysis. U87-MG whole cell extracts and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody (GTX105038) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DAG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105038

GTX105038 IHC-P Image

DAG1 antibody detects DAG1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
DAG1 stained by DAG1 antibody (GTX105038) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105038

GTX105038 ICC/IF Image

DAG1 antibody detects DAG1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DAG1 stained by DAG1 antibody (GTX105038) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DAG1 antibody used in Western Blot (WB). GTX105038

GTX105038 WB Image

MCF-7 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody (GTX105038) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DAG1 antibody used in Western Blot (WB). GTX105038

GTX105038 WB Image

293T whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody (GTX105038) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169