GeneTex
United States (US)

DAG1 antibody

Orthogonal
GTX105038_39876_20150410_IFA_19011506_534.jpg
GTX105038_39876_IHC_19011506_204.jpg
GTX105038_39876_20150903_WB_Fraction_19011506_812.jpg
GTX105038_39876_IFA_19011506_261.jpg

Cat No. GTX105038

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Horse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

U87-MG , U87-MG membrane fraction extract

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.66mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Dystroglycan. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

156DAG antibody, A3a antibody, AGRNR antibody, DAG antibody, DAG1 antibody, dystroglycan antibody, dystroglycan 1 (dystrophin-associated glycoprotein 1) antibody

Cellular Localization

Alpha-dystroglycan: Secreted , extracellular space , Beta-dystroglycan: Cell membrane , Cytoplasm , cytoskeleton

Background

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105038_39876_20150410_IFA_19011506_534.jpg

GTX105038 ICC/IF Image

alpha Dystroglycan antibody detects alpha Dystroglycan protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Dystroglycan protein stained by alpha Dystroglycan antibody (GTX105038) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX105038_39876_IHC_19011506_204.jpg

GTX105038 IHC-P Image

alpha Dystroglycan antibody detects DAG1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
alpha Dystroglycan antibody (GTX105038) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105038_39876_20150903_WB_Fraction_19011506_812.jpg

GTX105038 WB Image

Orthogonal

DAG1 antibody detects DAG1 protein by western blot analysis. U87-MG whole cell extracts and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody (GTX105038) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX105038_39876_IFA_19011506_261.jpg

GTX105038 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using alpha Dystroglycan(GTX105038) antibody at 1:500 dilution.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169
Current in productions