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DAG1 antibody

Anti-DAG1 antibody used in Western Blot (WB). GTX64471
Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64471
Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64471
Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64471

Cat. No. GTX64471

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DAG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:20 - 1:100
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 654-749 of human DAG1 (NP_004384.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dystroglycan 1 , 156DAG , A3a , AGRNR , DAG , LGMDR16 , MDDGA9 , MDDGC7 , MDDGC9

Cellular Localization

Cytoplasm, cytoskeleton,nucleoplasm

Background

This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-DAG1 antibody used in Western Blot (WB). GTX64471

GTX64471 WB Image

WB analysis of various sample lysates using GTX64471 DAG1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64471

GTX64471 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64471 DAG1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64471

GTX64471 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64471 DAG1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DAG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64471

GTX64471 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX64471 DAG1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399