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DAG1 / beta Dystroglycan antibody [C2C3-2], C-term

Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124225
Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124225
Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225

Cat No. GTX124225

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

H1299 , MCF-7

Predict Reactivity

Rat, Rabbit, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Human DAG1 / beta Dystroglycan. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dystroglycan 1 , 156DAG , A3a , AGRNR , DAG , LGMDR16 , MDDGA9 , MDDGC7 , MDDGC9

Cellular Localization

Alpha-dystroglycan: Secreted , extracellular space , Beta-dystroglycan: Cell membrane , Cytoplasm , cytoskeleton

Background

Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX124225

GTX124225 IHC-P Image

beta dystroglycan antibody [C2C3-2], C-term detects beta dystroglycan protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
beta dystroglycan antibody [C2C3-2], C-term (GTX124225) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124225

GTX124225 ICC/IF Image

beta dystroglycan antibody [C2C3-2], C-term detects beta dystroglycan protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta dystroglycan protein stained by beta dystroglycan antibody [C2C3-2], C-term (GTX124225) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-DAG1 / beta Dystroglycan antibody [C2C3-2], C-term used in Western Blot (WB). GTX124225

GTX124225 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAG1 / beta Dystroglycan antibody [C2C3-2], C-term (GTX124225) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for DAG1 / beta Dystroglycan antibody [C2C3-2], C-term (GTX124225). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DAG1 / beta Dystroglycan antibody [C2C3-2], C-term (GTX124225). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169