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DAG1 / beta Dystroglycan antibody [GT2812]

KO/KD
Anti-DAG1 / beta Dystroglycan antibody [GT2812] used in Western Blot (WB). GTX635180
Anti-DAG1 / beta Dystroglycan antibody [GT2812] used in Western Blot (WB). GTX635180

Cat. No. GTX635180

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT2812

Isotype

 IgG2a

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DAG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Product Note

This antibody was raised against human DAG1 / beta Dystroglycan Extracellular domain.

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human DAG1 / beta Dystroglycan. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888510

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dystroglycan 1 , 156DAG , A3a , AGRNR , DAG , LGMDR16 , MDDGA9 , MDDGC7 , MDDGC9

Cellular Localization

Cytoplasm, cytoskeleton,nucleoplasm

Background

This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]

Database

Research Area

DATA IMAGES

Anti-DAG1 / beta Dystroglycan antibody [GT2812] used in Western Blot (WB). GTX635180

GTX635180 WB Image

KO/KD

Wild-type (WT) and DAG1 / beta Dystroglycan knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAG1 / beta Dystroglycan antibody [GT2812] (GTX635180) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-DAG1 / beta Dystroglycan antibody [GT2812] used in Western Blot (WB). GTX635180

GTX635180 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAG1 / beta Dystroglycan antibody [GT2812] (GTX635180) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations