APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Product Note
This antibody was raised against human DAG1 / beta Dystroglycan Extracellular domain.
Predict Reactivity
Rat, Rabbit, Bovine, Cat, Dog, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human DAG1 / beta Dystroglycan. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888509
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
dystroglycan 1 , 156DAG , A3a , AGRNR , DAG , LGMDR16 , MDDGA9 , MDDGC7 , MDDGC9
Cellular Localization
Cytoplasm, cytoskeleton,nucleoplasm
Background
This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. [provided by RefSeq, Nov 2015]
Database
Research Area
DATA IMAGES
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179](/templates/default/js/lightbox/images/loading.gif "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179")
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GTX635179 WB Image
Wild-type (WT) and DAG1 / beta Dystroglycan knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAG1 / beta Dystroglycan antibody [GT835] (GTX635179) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX635179 ICC/IF Image
DAG1 / beta Dystroglycan antibody [GT835] detects DAG1 / beta Dystroglycan protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DAG1 / beta Dystroglycan stained by DAG1 / beta Dystroglycan antibody [GT835] (GTX635179) diluted at 1:100.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX635179 IHC-P Image
DAG1 / beta Dystroglycan antibody [GT835] detects DAG1 / beta Dystroglycan protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse placenta.DAG1 / beta Dystroglycan stained by DAG1 / beta Dystroglycan antibody [GT835] (GTX635179) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635179 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAG1 / beta Dystroglycan antibody [GT835] (GTX635179) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for DAG1 / beta Dystroglycan antibody [GT835] (GTX635179). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DAG1 / beta Dystroglycan antibody [GT835] (GTX635179). Be the first to share your experience with this product.
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179](/upload/website/prouct_img/small/GTX635179/GTX635179_43556_20190525_WB_KO_watermark_w_23061202_547.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179")
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635179](/upload/website/prouct_img/small/GTX635179/GTX635179_43556_20200212_ICC_IF_w_23061202_714.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635179")
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in IHC (Paraffin sections) (IHC-P). GTX635179](/upload/website/prouct_img/small/GTX635179/GTX635179_43556_20190614_IHC-P_M_1_w_23061202_497.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in IHC (Paraffin sections) (IHC-P). GTX635179")
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179](/upload/website/prouct_img/small/GTX635179/GTX635179_43556_20190510_WB_w_23061202_972.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179")
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179](/upload/website/prouct_img/normal/GTX635179/GTX635179_43556_20190525_WB_KO_watermark_w_23061202_547.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179")
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635179](/upload/website/prouct_img/normal/GTX635179/GTX635179_43556_20200212_ICC_IF_w_23061202_714.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635179")
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in IHC (Paraffin sections) (IHC-P). GTX635179](/upload/website/prouct_img/normal/GTX635179/GTX635179_43556_20190614_IHC-P_M_1_w_23061202_497.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in IHC (Paraffin sections) (IHC-P). GTX635179")
![Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179](/upload/website/prouct_img/normal/GTX635179/GTX635179_43556_20190510_WB_w_23061202_972.webp "Anti-DAG1 / beta Dystroglycan antibody [GT835] used in Western Blot (WB). GTX635179")