0.01M PBS pH7.4, 15 mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Phosphopeptide corresponding to amino acids 303-312 (pSer308) of human DAP-kinase, conjugated to KLH.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Various apoptotic signals use Death Associated Protein Kinase (DAPK) as a downstream effector in different cell types. DAPK is a positive mediator of apoptosis and is widely expressed in many tissues of embryonic and adult origin. DAPK is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active. DAPK activity is also regulated by phosphorylation. DAPK was found to be negatively regulated by autophosphorylation at Ser308 which is in the calmodulin regulatory domain. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. As a consequence, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal antibodies specific for Phospho DAP-Kinase (pSer308) are important tools for studying the mechanism of DAPK activation in apoptosis.