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DAP Kinase 2 antibody [1C5]

Overexpression
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in Western Blot (WB). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in Immunoprecipitation (IP). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in Western Blot (WB). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in Flow cytometry (FACS). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in Flow cytometry (FACS). GTX84631
Anti-DAP Kinase 2 antibody [1C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84631

Cat. No. GTX84631

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1C5

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all DAP Kinase 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
IP 2ug/500ul
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

death associated protein kinase 2 , DRP-1 , DRP1

Cellular Localization

Cytoplasm

Background

Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.

Database

Research Area

DATA IMAGES

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human liver tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in Western Blot (WB). GTX84631

GTX84631 WB Image

WB analysis of various cell lines using GTX84631 DAP Kinase 2 antibody [1C5].
Loading : 35 ug per lane

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human lung tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human colon tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in IHC (Paraffin sections) (IHC-P). GTX84631

GTX84631 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84631 DAP Kinase 2 antibody [1C5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-DAP Kinase 2 antibody [1C5] used in Immunoprecipitation (IP). GTX84631

GTX84631 IP Image

IP analysis of DDDDK tagged DAP Kinase 2 overexpressed HEK293T lysate using GTX84631 DAP Kinase 2 antibody [1C5].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-DAP Kinase 2 antibody [1C5] used in Western Blot (WB). GTX84631

GTX84631 WB Image

Overexpression

WB analysis of HEK293T cells transfected with DAP Kinase 2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84631 DAP Kinase 2 antibody [1C5].
Loading : 5 ug per lane

Anti-DAP Kinase 2 antibody [1C5] used in Flow cytometry (FACS). GTX84631

GTX84631 FACS Image

FACS analysis of Jurkat cells using GTX84631 DAP Kinase 2 antibody [1C5].
Red : Primary antibody
Blue : Negative control antibody

Anti-DAP Kinase 2 antibody [1C5] used in Flow cytometry (FACS). GTX84631

GTX84631 FACS Image

FACS analysis of HEK293T cells transfected with either DAP Kinase 2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84631 DAP Kinase 2 antibody [1C5].

Anti-DAP Kinase 2 antibody [1C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84631

GTX84631 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with DAP Kinase 2 plasmid using GTX84631 DAP Kinase 2 antibody [1C5].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519