GeneTex
United States (US)

DAP Kinase 2 antibody

DAP-Kinase-2-antibody_IHC-P_GTX28404-1_18121409_502.jpg
DAP-Kinase-2-antibody_WB_GTX28404-1_18121409_810.jpg

Cat No. GTX28404

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

43 kDa. ( Note )

Positive Control

A431 whole cell lysate

Specificity/Sensitivity

Does not react with DAPK1
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide - amino acids 356-370 of human DAPK2. This sequence is identical to that of mouse.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Death Associated Protein Kinase 2,Drp-1,Drp1,Dapk2

Background

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase-containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNg induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1 (1, 2). Ectopicly expressed DAPK2 induced apoptosis in various types of cells (1,2). DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA ofDAPK2 is expressed in multiple human tissues (1).

Database

Research Area

DATA IMAGES
DAP-Kinase-2-antibody_IHC-P_GTX28404-1_18121409_502.jpg

GTX28404 IHC-P Image

Immunohistochemical staining of DAPK2 in mouse spleen cells with DAPK2 antibody (GTX28404) at 2μg/ml.

DAP-Kinase-2-antibody_WB_GTX28404-1_18121409_810.jpg

GTX28404 WB Image

Western blot analysis of DAPK2 in A431 (H), mouse spleen (M), and rat kidney (R) lysates with DAPK2 antibody (GTX28404) at 1μg/ml.

Package List Price ($)
$ 329