GeneTex
United States (US)

DAP Kinase 3 antibody

GTX102404_39820_WB_19011506_504.jpg
GTX102404_39820_20150410_IFA_19011506_529.jpg

Cat No. GTX102404

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Bovine, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DAP Kinase 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Death Associated Protein Kinase 3,Dlk,Zip,Zipk,Dapk3

Cellular Localization

Nucleus , Cytoplasm

Background

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102404_39820_WB_19011506_504.jpg

GTX102404 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX102404 diluted at 1:500

GTX102404_39820_20150410_IFA_19011506_529.jpg

GTX102404 ICC/IF Image

DAP Kinase 3 antibody detects DAP Kinase 3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DAP Kinase 3 protein stained by DAP Kinase 3 antibody (GTX102404) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169