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DAP Kinase 3 antibody

Orthogonal
Anti-DAP Kinase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102404
Anti-DAP Kinase 3 antibody used in Western Blot (WB). GTX102404
Anti-DAP Kinase 3 antibody used in Western Blot (WB). GTX102404

Cat. No. GTX102404

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DAP Kinase 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Bovine, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DAP Kinase 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036736

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

death associated protein kinase 3 , DLK , ZIP , ZIPK

Cellular Localization

Nucleus , Cytoplasm

Background

Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DAP Kinase 3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102404

GTX102404 ICC/IF Image

DAP Kinase 3 antibody detects DAP Kinase 3 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DAP Kinase 3 protein stained by DAP Kinase 3 antibody (GTX102404) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Anti-DAP Kinase 3 antibody used in Western Blot (WB). GTX102404

GTX102404 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX102404 diluted at 1:500

Anti-DAP Kinase 3 antibody used in Western Blot (WB). GTX102404

GTX102404 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

REFERENCE

Application Reference

Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Liu YH et al. Sci Rep 2017; 7 : 41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Fujiwara N et al. J Biol Chem 2016; 291 (20) : 10858-66 Regulation of Beclin 1 Protein Phosphorylation and Autophagy by Protein Phosphatase 2A (PP2A) and Death-associated Protein Kinase 3 (DAPK3).
Application : WB
Reactivity : Mouse
Kameshima S et al. Biochem Biophys Res Commun 2016; 469 (3) : 654-8 Expression and localization of calmodulin-related proteins in brain, heart and kidney from spontaneously hypertensive rats.
Application : WB
Reactivity : Rat
Satoshi Kameshima et al. Biochemical and Biophysical Research Communications 2015; Expression and localization of calmodulin-related proteins in brain, heart and kidney from spontaneously hypertensive rats.
Application : WB
Reactivity : Rat
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169