APPLICATION
Application Note
IP: Use at a concentration of 2-4 μg/mg of protein lysate. WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
NKp44 positive cells.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.08% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full length protein (Human).
Purification
IgG fraction
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
TYRO protein tyrosine kinase binding protein , DAP12 , KARAP , PLOSL , PLOSL1
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Mar 2010]
Database
Research Area
DATA IMAGES
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GTX16462 IP Image
GTX16462 at 3g (per mg of protein lysate)detecting DAP12 on MHC ClassI postive cells (Lane 1) and NKp44 postive cells (Lane 2) by immunoprecipitation.
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REFERENCE
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REVIEW
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