GeneTex
United States (US)

DAP12 antibody

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Cat No. GTX16462

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

IP: Use at a concentration of 2-4 μg/mg of protein lysate. WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

12 kDa. ( Note )

Positive Control

NKp44 positive cells.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length protein (Human).

Purification

IgG fraction

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyro Protein Tyrosine Kinase Binding Protein,Dap12,Karap,Plosl,Tyrobp

Cellular Localization

Plasma membrane

Background

DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells. DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction. Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice, as well as presenile dementia and bone cysts in humans.

Database

Research Area

DATA IMAGES
img26013_18121411_368.jpg

GTX16462 IP Image

GTX16462 at 3g (per mg of protein lysate)detecting DAP12 on MHC ClassI postive cells (Lane 1) and NKp44 postive cells (Lane 2) by immunoprecipitation.

Package List Price ($)
$ 389