APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 5μg/ml |
| 2.5μg/ml |
|
Note :
ICC/IF
Protein expression clearly seen within the nucleus.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89921-PEP).
Product Note
This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1; NP_001186779.1; NP_001186780.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
death associated protein 3 , DAP-3 , MRP-S29 , MRPS29 , S29mt , bMRP-10
Cellular Localization
Mitochondrion
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]
Database
Research Area
DATA IMAGES
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GTX89921 IHC-P Image
IHC-P analysis of human tonsil using GTX89921 DAP3 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89921 IHC-P Image
IHC-P analysis of human cortex using GTX89921 DAP3 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
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GTX89921 WB Image
WB analysis of HeLa lysate using GTX89921 DAP3 antibody, C-term.
Dilution : 3μg/ml
Loading : 30μg protein in RIPA buffer
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GTX89921 ICC/IF Image
ICC/IF analysis of guanidinium thiocyanate-treated HeLa control (left) and after (right) si-RNA-mediated DAP3 knock-down cells using GTX89921 DAP3 antibody, C-term.
Dilution : 0.5μg/ml
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GTX89921 WB Image
WB analysis of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) using GTX89921 DAP3 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89921 ICC/IF Image
ICC/IF analysis of MCF7 cells using GTX89921 DAP3 antibody, C-term.
Dilution : 5μg/ml
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REFERENCE
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REVIEW
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