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DAP3 antibody, C-term

Anti-DAP3 antibody, C-term used in Western Blot (WB). GTX89921
Anti-DAP3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89921
Anti-DAP3 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89921
Anti-DAP3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89921
Anti-DAP3 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89921
Anti-DAP3 antibody, C-term used in Western Blot (WB). GTX89921

Cat No. GTX89921

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
ICC/IF 5μg/ml
IHC-P 2.5μg/ml

Note :

ICC/IF
Protein expression clearly seen within the nucleus.

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89921-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1).

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1; NP_001186779.1; NP_001186780.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

death associated protein 3 , DAP-3 , MRP-S29 , MRPS29 , S29mt , bMRP-10

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-DAP3 antibody, C-term used in Western Blot (WB). GTX89921

GTX89921 WB Image

WB analysis of HeLa lysate using GTX89921 DAP3 antibody, C-term.
Dilution : 3μg/ml
Loading : 30μg protein in RIPA buffer

Anti-DAP3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89921

GTX89921 IHC-P Image

IHC-P analysis of human tonsil using GTX89921 DAP3 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-DAP3 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89921

GTX89921 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX89921 DAP3 antibody, C-term.
Dilution : 5μg/ml

Anti-DAP3 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89921

GTX89921 IHC-P Image

IHC-P analysis of human cortex using GTX89921 DAP3 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-DAP3 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89921

GTX89921 ICC/IF Image

ICC/IF analysis of guanidinium thiocyanate-treated HeLa control (left) and after (right) si-RNA-mediated DAP3 knock-down cells using GTX89921 DAP3 antibody, C-term.
Dilution : 0.5μg/ml

Anti-DAP3 antibody, C-term used in Western Blot (WB). GTX89921

GTX89921 WB Image

WB analysis of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) using GTX89921 DAP3 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

There are currently no references for DAP3 antibody, C-term (GTX89921). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DAP3 antibody, C-term (GTX89921). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 369