Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAP3 antibody [N1C2] (GTX113863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113863 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DAP3(GTX113863) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using DAP3(GTX113863) antibody at 1:200 dilution.