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DAP3 antibody [N1C2]

Anti-DAP3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113863
Anti-DAP3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113863
Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863
Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863

Cat. No. GTX113863

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DAP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DAP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036734

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

death associated protein 3 , DAP-3 , MRP-S29 , MRPS29 , S29mt , bMRP-10

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DAP3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113863

GTX113863 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DAP3(GTX113863) antibody at 1:200 dilution.

Anti-DAP3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113863

GTX113863 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DAP3(GTX113863) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863

GTX113863 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113863 diluted at 1:1000

Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863

GTX113863 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAP3 antibody [N1C2] (GTX113863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169