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DAP3 antibody [N1C2]

Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863
Anti-DAP3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113863
Anti-DAP3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113863
Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863

Cat No. GTX113863

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DAP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036734

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

death associated protein 3 , DAP-3 , MRP-S29 , MRPS29 , S29mt , bMRP-10

Cellular Localization

Mitochondrion

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863

GTX113863 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX113863 diluted at 1:1000

Anti-DAP3 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX113863

GTX113863 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DAP3(GTX113863) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DAP3 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113863

GTX113863 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DAP3(GTX113863) antibody at 1:200 dilution.

Anti-DAP3 antibody [N1C2] used in Western Blot (WB). GTX113863

GTX113863 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DAP3 antibody [N1C2] (GTX113863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for DAP3 antibody [N1C2] (GTX113863). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DAP3 antibody [N1C2] (GTX113863). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169