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DAP5 antibody [39C534.1]

Anti-DAP5 antibody [39C534.1] used in Flow cytometry (FACS). GTX11929
Anti-DAP5 antibody [39C534.1] used in Western Blot (WB). GTX11929

Cat. No. GTX11929

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 39C534.1

Isotype

 IgG1

Application

 WB, FACS

Reactivity

 Human, Mouse, Hamster
Package
50 μg ($399)
See all DAP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
FACS 1 μg / 10⁶ cells
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% Gelatin

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antibody was raised against two synthetic peptides corresponding to amino acids 796-812 of human DAP-5 protein.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4 gamma 2 , AAG1 , DAP5 , NAT1 , P97

Background

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DAP5 antibody [39C534.1] used in Flow cytometry (FACS). GTX11929

GTX11929 FACS Image

FACS (Intracellular staining) analysis of CHO (A) and HEK293 (B) cells using GTX11929 DAP5 antibody [39C534.1].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/1 x 10⁶ cells

Anti-DAP5 antibody [39C534.1] used in Western Blot (WB). GTX11929

GTX11929 WB Image

WB analysis of Jurkat cell lysate using GTX11929 DAP5 antibody [39C534.1].
Loading : 15μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399