APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , HeLa , HepG2
Predict Reactivity
Rat, Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DAP5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240756
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 4 gamma 2 , AAG1 , DAP5 , NAT1 , P97
Background
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712](/templates/default/js/lightbox/images/loading.gif "Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712")
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GTX101712 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAP5 antibody [C2C3], C-term (GTX101712) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101712 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAP5 antibody [C2C3], C-term (GTX101712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101712 IHC-P Image
DAP5 antibody [C2C3], C-term detects EIF4G2 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
DAP5 antibody [C2C3], C-term (GTX101712) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101712 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101712 diluted at 1:500
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GTX101712 IHC-P Image
DAP5 antibody [C2C3], C-term detects DAP5 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DAP5 antibody [C2C3], C-term (GTX101712) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for DAP5 antibody [C2C3], C-term (GTX101712). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DAP5 antibody [C2C3], C-term (GTX101712). Be the first to share your experience with this product.
![Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712](/upload/website/prouct_img/small/GTX101712/GTX101712_39721_20230929_WB_B_23100319_875.webp "Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712](/upload/website/prouct_img/small/GTX101712/GTX101712_39721_20231013_WB_23101921_410.webp "Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712](/upload/website/prouct_img/small/GTX101712/GTX101712_39721_IHC_w_23060100_503.webp "Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712](/upload/website/prouct_img/small/GTX101712/GTX101712_39721_WB_M_brain_w_23060100_975.webp "Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712](/upload/website/prouct_img/small/GTX101712/GTX101712_39721_IHC_M_w_23060100_883.webp "Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712](/upload/website/prouct_img/normal/GTX101712/GTX101712_39721_20230929_WB_B_23100319_875.webp "Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712](/upload/website/prouct_img/normal/GTX101712/GTX101712_39721_20231013_WB_23101921_410.webp "Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712](/upload/website/prouct_img/normal/GTX101712/GTX101712_39721_IHC_w_23060100_503.webp "Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712](/upload/website/prouct_img/normal/GTX101712/GTX101712_39721_WB_M_brain_w_23060100_975.webp "Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712")
![Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712](/upload/website/prouct_img/normal/GTX101712/GTX101712_39721_IHC_M_w_23060100_883.webp "Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712")