GeneTex
United States (US)

DAP5 antibody [C2C3], C-term

Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712
Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712
Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712
Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712

Cat No. GTX101712

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

HeLa , HepG2 , mouse brain

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DAP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4 Gamma 2,Aag1,Dap5,Nat1,P97,Eif4G2

Background

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712

GTX101712 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX101712 diluted at 1:1000

Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712

GTX101712 IHC-P Image

DAP5 antibody [C2C3], C-term detects DAP5 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DAP5 antibody [C2C3], C-term (GTX101712) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DAP5 antibody [C2C3], C-term used in Western Blot (WB). GTX101712

GTX101712 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101712 diluted at 1:500

Anti-DAP5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101712

GTX101712 IHC-P Image

DAP5 antibody [C2C3], C-term detects EIF4G2 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
DAP5 antibody [C2C3], C-term (GTX101712) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169