Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAP5 antibody [C2C3], C-term (GTX101712) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAP5 antibody [C2C3], C-term (GTX101712) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DAP5 antibody [C2C3], C-term detects DAP5 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DAP5 antibody [C2C3], C-term (GTX101712) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101712 diluted at 1:500

DAP5 antibody [C2C3], C-term detects EIF4G2 protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
DAP5 antibody [C2C3], C-term (GTX101712) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min