Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAP5 antibody (GTX101710) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EIF4G2 (GTX101710) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

DAP5 antibody detects EIF4G2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EIF4G2 protein stained by DAP5 antibody (GTX101710) diluted at 1:500.
Blue: Hoechst 33343 staining.

DAP5 antibody detects DAP5 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DAP5 antibody (GTX101710) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101710 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded human skeletal muscle , using DAP5(GTX101710) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX101710 diluted at 1:1000