APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , Mouse brain
Predict Reactivity
Rat, Rabbit, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 40% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human DAP5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240755
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 4 gamma 2 , AAG1 , DAP5 , NAT1 , P97
Background
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101710 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAP5 antibody (GTX101710) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101710 WB Image
Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX101710 diluted at 1:1000
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GTX101710 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human skeletal muscle , using DAP5(GTX101710) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101710 WB Image
Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101710 diluted at 1:500
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GTX101710 IHC-P Image
DAP5 antibody detects DAP5 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DAP5 antibody (GTX101710) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101710 ICC/IF Image
DAP5 antibody detects EIF4G2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EIF4G2 protein stained by DAP5 antibody (GTX101710) diluted at 1:500.
Blue: Hoechst 33343 staining.
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GTX101710 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EIF4G2 (GTX101710) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
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REVIEW
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