GeneTex
United States (US)

DAP5 antibody

GTX101710_41052_IHC_M_19011506_876.jpg
GTX101710_39596_IFA_19011506_857.jpg
GTX101710_IHC_19011506_357.jpg
GTX101710_39596_IHC2_19011506_834.jpg
GTX101710_WB_19011506_292.jpg
GTX101710_39596_WB_M_brain_19011506_921.jpg

Cat No. GTX101710

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

A431 , mouse brain

Predict Reactivity

Rat, Rabbit, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DAP5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4 Gamma 2,Aag1,Dap5,Nat1,P97,Eif4G2

Background

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101710_41052_IHC_M_19011506_876.jpg

GTX101710 IHC-P Image

DAP5 antibody detects DAP5 protein at cytosol on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DAP5 antibody (GTX101710) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101710_39596_IFA_19011506_857.jpg

GTX101710 ICC/IF Image

DAP5 antibody detects EIF4G2 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EIF4G2 protein stained by DAP5 antibody (GTX101710) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX101710_IHC_19011506_357.jpg

GTX101710 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EIF4G2 (GTX101710) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101710_39596_IHC2_19011506_834.jpg

GTX101710 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human skeletal muscle , using DAP5(GTX101710) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101710_WB_19011506_292.jpg

GTX101710 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX101710 diluted at 1:1000

GTX101710_39596_WB_M_brain_19011506_921.jpg

GTX101710 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX101710 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169