GeneTex
United States (US)

DAP5 antibody

GTX33144_20180530_IHC-P_3_18121410_598.jpg
GTX33144_20180530_IHC-P_2_18121410_924.jpg
GTX33144_20180530_WB_18121410_826.jpg
GTX33144_20180530_IHC-P_1_18121410_578.jpg
GTX33144_20180530_IHC-P_18121410_505.jpg

Cat No. GTX33144

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 750-850 of human EIF4G2 (NP_001409.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Eukaryotic Translation Initiation Factor 4 Gamma 2,Aag1,Dap5,Nat1,P97,Eif4G2

Background

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX33144_20180530_IHC-P_3_18121410_598.jpg

GTX33144 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX33144 DAP5 antibody.

GTX33144_20180530_IHC-P_2_18121410_924.jpg

GTX33144 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX33144 DAP5 antibody.

GTX33144_20180530_WB_18121410_826.jpg

GTX33144 WB Image

WB analysis of various samples using GTX33144 DAP5 antibody.
Dilution : 1:400
Loading : 25μg per lane

GTX33144_20180530_IHC-P_1_18121410_578.jpg

GTX33144 IHC-P Image

IHC-P analysis of human stomach tissue using GTX33144 DAP5 antibody.

GTX33144_20180530_IHC-P_18121410_505.jpg

GTX33144 IHC-P Image

IHC-P analysis of human liver injury tissue using GTX33144 DAP5 antibody.

Package List Price ($)
$ 329