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DAP5 antibody

Anti-DAP5 antibody used in Western Blot (WB). GTX33144
Anti-DAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33144
Anti-DAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33144
Anti-DAP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33144
Anti-DAP5 antibody used in Immunoprecipitation (IP). GTX33144
Anti-DAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33144

Cat. No. GTX33144

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DAP5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:200
IHC-P 1:100 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 750-850 of human EIF4G2 (NP_001409.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

eukaryotic translation initiation factor 4 gamma 2 , AAG1 , DAP5 , NAT1 , P97

Background

Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DAP5 antibody used in Western Blot (WB). GTX33144

GTX33144 WB Image

WB analysis of various sample lysates using GTX33144 DAP5 antibody.
Dilution : 1:400
Loading : 25μg per lane

Anti-DAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33144

GTX33144 ICC/IF Image

ICC/IF analysis of C6 cells using GTX33144 DAP5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33144

GTX33144 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33144 DAP5 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DAP5 antibody used in IHC (Paraffin sections) (IHC-P). GTX33144

GTX33144 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33144 DAP5 antibody.
Dilution : 1:100

Anti-DAP5 antibody used in Immunoprecipitation (IP). GTX33144

GTX33144 IP Image

IP analysis of HeLa cell lysate using GTX33144 DAP5 antibody.
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-DAP5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33144

GTX33144 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33144 DAP5 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399