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DARPP-32 antibody [SU0329]

Recombinant antibody
Anti-DARPP-32 antibody [SU0329] used in Western Blot (WB). GTX01132
Anti-DARPP-32 antibody [SU0329] used in Western Blot (WB). GTX01132
Anti-DARPP-32 antibody [SU0329] used in Western Blot (WB). GTX01132
Anti-DARPP-32 antibody [SU0329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01132
Anti-DARPP-32 antibody [SU0329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01132
Anti-DARPP-32 antibody [SU0329] used in IHC (Paraffin sections) (IHC-P). GTX01132
Anti-DARPP-32 antibody [SU0329] used in IHC (Paraffin sections) (IHC-P). GTX01132
Anti-DARPP-32 antibody [SU0329] used in Flow cytometry (FACS). GTX01132
Anti-DARPP-32 antibody [SU0329] used in IHC (Paraffin sections) (IHC-P). GTX01132

Cat No. GTX01132

Host

Rabbit

Clonality

Monoclonal

Clone Name

SU0329

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse, Rat
Package
100 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
FACS 1:50-1:100
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Observed MW

~32 kDa. The observed M.W. is based on the following publication. PMID: 9334390

PROPERTIES

Form

Liquid

Buffer

1x TBS (pH7.4), 1% BSA, 40% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 1 regulatory inhibitor subunit 1B , DARPP32 , PPP1R1B , DARPP-32

Cellular Localization

Cytoplasm

Background

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-DARPP-32 antibody [SU0329] used in Western Blot (WB). GTX01132

GTX01132 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DARPP-32 antibody [SU0329] (GTX01132) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DARPP-32 antibody [SU0329] used in Western Blot (WB). GTX01132

GTX01132 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DARPP-32 antibody [SU0329] (GTX01132) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-DARPP-32 antibody [SU0329] used in Western Blot (WB). GTX01132

GTX01132 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DARPP-32 antibody [SU0329] (GTX01132) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication:PMID: 9334390

Anti-DARPP-32 antibody [SU0329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01132

GTX01132 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX01132 DARPP-32 antibody [SU0329].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-DARPP-32 antibody [SU0329] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01132

GTX01132 ICC/IF Image

ICC/IF analysis of PFA-fixed SH-SY-5Y cells using GTX01132 DARPP-32 antibody [SU0329].
Green : primary antibody
Blue : DAPI
Permeabilization : 0.25% Triton X-100 in PBS

Anti-DARPP-32 antibody [SU0329] used in IHC (Paraffin sections) (IHC-P). GTX01132

GTX01132 IHC-P Image

IHC-P analysis of human gastric carcinoma tissue section using GTX01132 DARPP-32 antibody [SU0329].

Anti-DARPP-32 antibody [SU0329] used in IHC (Paraffin sections) (IHC-P). GTX01132

GTX01132 IHC-P Image

IHC-P analysis of human breast carcinoma tissue section using GTX01132 DARPP-32 antibody [SU0329].

Anti-DARPP-32 antibody [SU0329] used in Flow cytometry (FACS). GTX01132

GTX01132 FACS Image

FACS analysis of HeLa cells using GTX01132 DARPP-32 antibody [SU0329].
Red : primary antibody
Black : unlabelled control
Dilution : 1:50

Anti-DARPP-32 antibody [SU0329] used in IHC (Paraffin sections) (IHC-P). GTX01132

GTX01132 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX01132 DARPP-32 antibody [SU0329].

REFERENCE

There are currently no references for DARPP-32 antibody [SU0329] (GTX01132). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DARPP-32 antibody [SU0329] (GTX01132). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Sodium Azide.pdf