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DARPP-32 antibody

Anti-DARPP-32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133336
Anti-DARPP-32 antibody used in Western Blot (WB). GTX133336
Anti-DARPP-32 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133336

Cat. No. GTX133336

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DARPP-32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse DARPP-32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886937

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1, regulatory inhibitor subunit 1B , AU040756 , DARPP-32 , Darpp32

Cellular Localization

Cytoplasm

Background

Inhibitor of protein-phosphatase 1.

Database

Research Area

DATA IMAGES

Anti-DARPP-32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133336

GTX133336 ICC/IF Image

DARPP-32 antibody detects DARPP-32 protein at cell body by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DARPP-32 protein stained by DARPP-32 antibody (GTX133336) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-DARPP-32 antibody used in Western Blot (WB). GTX133336

GTX133336 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DARPP-32 antibody (GTX133336) diluted at 1:5000.

Anti-DARPP-32 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133336

GTX133336 IHC-Fr Image

DARPP-32 antibody detects DARPP-32 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: DARPP-32 protein stained by DARPP-32 antibody (GTX133336) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169