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DARPP-32 antibody

Anti-DARPP-32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133350
Anti-DARPP-32 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133350
Anti-DARPP-32 antibody used in Western Blot (WB). GTX133350
Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350

Cat No. GTX133350

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.91 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse DARPP-32. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein phosphatase 1, regulatory inhibitor subunit 1B , AU040756 , DARPP-32 , Darpp32

Cellular Localization

Cytoplasm

Background

Inhibitor of protein-phosphatase 1.

Database

Research Area

DATA IMAGES

Anti-DARPP-32 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133350

GTX133350 ICC/IF Image

DARPP-32 antibody detects DARPP-32 protein at cell body by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DARPP-32 protein stained by DARPP-32 antibody (GTX133350) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Fluoroshield with DAPI (GTX30920).

Anti-DARPP-32 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133350

GTX133350 IHC-Fr Image

DARPP-32 antibody detects DARPP-32 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: DARPP-32 protein stained by DARPP-32 antibody (GTX133350) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DARPP-32 antibody used in Western Blot (WB). GTX133350

GTX133350 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DARPP-32 antibody (GTX133350) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 22593050

Anti-DARPP-32 antibody used in IHC (Paraffin sections) (IHC-P). GTX133350

GTX133350 IHC-P Image

DARPP-32 antibody detects DARPP-32 protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
DARPP-32 antibody (GTX133350) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for DARPP-32 antibody (GTX133350). Be the first to share your publications with this product.

REVIEW

DARPP-32 antibody Cat No. GTX133350

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Frozen sections)(IHC-Fr) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 22-Mar-2018
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse brain (striatum)
Fixation : 4%PFA
Permeabilization /Antigen retrieval : PBST (0.3% TritonX-100)
Primary Antibody : 1:500, 25°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169