APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34.
Purification
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
DARPP32 , PPP1R1B , protein phosphatase 1 regulatory inhibitor subunit 1B , DARPP-32
Cellular Localization
Cytoplasm
Background
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Database
Research Area
DATA IMAGES
|
GTX00802 IHC-P Image
IHC-P analysis of human colon cancer tissue sections using GTX00802 DARPP-32 (phospho Thr34) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX00802 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00802 DARPP-32 (phospho Thr34) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX00802 WB Image
WB analysis of rat kidney and mouse brain tissue lysates using GTX00802 DARPP-32 (phospho Thr34) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
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GTX00802 ICC/IF Image
ICC/IF analysis of PFA-fixed MKN-45 cells treated with 30 μM Forskolin for 20 min using GTX00802 DARPP-32 (phospho Thr34) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400
|
REFERENCE
There are currently no references for DARPP-32 (phospho Thr34) antibody (GTX00802). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DARPP-32 (phospho Thr34) antibody (GTX00802). Be the first to share your experience with this product.
