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DARPP-32 (phospho Thr34) antibody

Anti-DARPP-32 (phospho Thr34) antibody used in IHC (Paraffin sections) (IHC-P). GTX00802
Anti-DARPP-32 (phospho Thr34) antibody used in ELISA (ELISA). GTX00802
Anti-DARPP-32 (phospho Thr34) antibody used in Western Blot (WB). GTX00802
Anti-DARPP-32 (phospho Thr34) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00802

Cat No. GTX00802

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr34.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DARPP32 , PPP1R1B , protein phosphatase 1 regulatory inhibitor subunit 1B , DARPP-32

Cellular Localization

Cytoplasm

Background

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-DARPP-32 (phospho Thr34) antibody used in IHC (Paraffin sections) (IHC-P). GTX00802

GTX00802 IHC-P Image

IHC-P analysis of human colon cancer tissue sections using GTX00802 DARPP-32 (phospho Thr34) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-DARPP-32 (phospho Thr34) antibody used in ELISA (ELISA). GTX00802

GTX00802 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00802 DARPP-32 (phospho Thr34) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-DARPP-32 (phospho Thr34) antibody used in Western Blot (WB). GTX00802

GTX00802 WB Image

WB analysis of rat kidney and mouse brain tissue lysates using GTX00802 DARPP-32 (phospho Thr34) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-DARPP-32 (phospho Thr34) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00802

GTX00802 ICC/IF Image

ICC/IF analysis of PFA-fixed MKN-45 cells treated with 30 μM Forskolin for 20 min using GTX00802 DARPP-32 (phospho Thr34) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400

REFERENCE

There are currently no references for DARPP-32 (phospho Thr34) antibody (GTX00802). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf