DARPP-32 (phospho Thr75) antibody

Cat. No. GTX00803

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all DARPP-32 products

Datasheet
Document

View product citations for antibody GTX00803 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
ICC/IF	1:100-1:500
IHC-P	1:50-1:200
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DARPP-32 around the phosphorylation site of Thr75.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DARPP32 , PPP1R1B , protein phosphatase 1 regulatory inhibitor subunit 1B , DARPP-32

Cellular Localization

Cytoplasm

Background

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Gene ID: 84152 PPP1R1B
UniProt: Q9UD71 PPP1R1B

Research Area

DATA IMAGES

GTX00803 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00803 DARPP-32 (phospho Thr75) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

GTX00803 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX00803 DARPP-32 (phospho Thr75) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

GTX00803 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00803 DARPP-32 (phospho Thr75) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

GTX00803 WB Image

WB analysis of mouse liver and rat brain tissue lysates using GTX00803 DARPP-32 (phospho Thr75) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty