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DARPP-32 (phospho Thr75) antibody

Anti-DARPP-32 (phospho Thr75) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50232

Cat. No. GTX50232

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all DARPP-32 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 75 (A-Y-T(p)-P-P) derived from human DARPP-32.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 regulatory inhibitor subunit 1B , DARPP-32 , DARPP32

Cellular Localization

Cytoplasm

Background

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-DARPP-32 (phospho Thr75) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50232

GTX50232 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50232 DARPP-32 (phospho Thr75) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399