Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using DARS(GTX103374) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed A431, using DARS(GTX103374) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
C: Raji
7.5% SDS PAGE
GTX103374 diluted at 1:1000

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DARS antibody [N1C1] (GTX103374) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.