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DARS antibody [N1C1]

Anti-DARS antibody [N1C1] used in Western Blot (WB). GTX103374
Anti-DARS antibody [N1C1] used in Western Blot (WB). GTX103374
Anti-DARS antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103374
Anti-DARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103374

Cat. No. GTX103374

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036737

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aspartyl-tRNA synthetase , HBSL , aspRS

Background

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DARS antibody [N1C1] used in Western Blot (WB). GTX103374

GTX103374 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DARS antibody [N1C1] (GTX103374) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DARS antibody [N1C1] used in Western Blot (WB). GTX103374

GTX103374 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
C: Raji
7.5% SDS PAGE
GTX103374 diluted at 1:1000

Anti-DARS antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103374

GTX103374 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using DARS(GTX103374) antibody at 1:200 dilution.

Anti-DARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103374

GTX103374 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using DARS(GTX103374) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169