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DARS antibody [N1C1]

Anti-DARS antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103374
Anti-DARS antibody [N1C1] used in Western Blot (WB). GTX103374
Anti-DARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103374

Cat No. GTX103374

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DARS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

aspartyl-tRNA synthetase , HBSL , aspRS

Background

Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis. [provided by RefSeq]

Database

DATA IMAGES
Anti-DARS antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103374

GTX103374 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using DARS(GTX103374) antibody at 1:200 dilution.

Anti-DARS antibody [N1C1] used in Western Blot (WB). GTX103374

GTX103374 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
B: Molt-4 (GTX27912)
C: Raji
7.5% SDS PAGE
GTX103374 diluted at 1:1000

Anti-DARS antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103374

GTX103374 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using DARS(GTX103374) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169