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DARS antibody

Anti-DARS antibody used in Western Blot (WB). GTX33145
Anti-DARS antibody used in Immunoprecipitation (IP). GTX33145
Anti-DARS antibody used in IHC (Paraffin sections) (IHC-P). GTX33145
Anti-DARS antibody used in IHC (Paraffin sections) (IHC-P). GTX33145
Anti-DARS antibody used in IHC (Paraffin sections) (IHC-P). GTX33145

Cat. No. GTX33145

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DARS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aspartyl-tRNA synthetase , HBSL , aspRS

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Database

Research Area

DATA IMAGES

Anti-DARS antibody used in Western Blot (WB). GTX33145

GTX33145 WB Image

WB analysis of various sample lysates using GTX33145 DARS antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DARS antibody used in Immunoprecipitation (IP). GTX33145

GTX33145 IP Image

IP analysis of 293T cell lysate using GTX33145 DARS antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-DARS antibody used in IHC (Paraffin sections) (IHC-P). GTX33145

GTX33145 IHC-P Image

IHC-P analysis of rat brain tissue using GTX33145 DARS antibody.
Dilution : 1:100

Anti-DARS antibody used in IHC (Paraffin sections) (IHC-P). GTX33145

GTX33145 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33145 DARS antibody.
Dilution : 1:100

Anti-DARS antibody used in IHC (Paraffin sections) (IHC-P). GTX33145

GTX33145 IHC-P Image

IHC-P analysis of mouse spinal cord tissue using GTX33145 DARS antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399