GeneTex
United States (US)

DAZ3 antibody

GTX106859_WB_19011506_142.jpg
GTX106859_39981_IHC_19011506_633.jpg
GTX106859_39981_IFA_19011506_838.jpg

Cat No. GTX106859

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HeLa
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DAZ3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC126441 antibody, pDP1679 antibody, DAZ3 antibody, deleted in azoospermia protein 3 antibody, deleted in azoospermia 3 antibody

Cellular Localization

Cytoplasm , Nucleus

Background

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. [provided by RefSeq]
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Database

DATA IMAGES
GTX106859_WB_19011506_142.jpg

GTX106859 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX106859 diluted at 1:500

GTX106859_39981_IHC_19011506_633.jpg

GTX106859 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DAZ3(GTX106859) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106859_39981_IFA_19011506_838.jpg

GTX106859 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAZ3(GTX106859) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169