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DAZ4 antibody, Internal

Anti-DAZ4 antibody, Internal used in Western Blot (WB). GTX47420

Cat No. GTX47420

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
50 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

HT1080

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human DAZ4

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

deleted in azoospermia 4 , pDP1680 , pDP1681

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains two copies of the 10.8 kb repeat. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2011]

Database

DATA IMAGES

Anti-DAZ4 antibody, Internal used in Western Blot (WB). GTX47420

GTX47420 WB Image

WB analysis of HT1080 cells using GTX47420 DAZ4 antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329