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DAZL antibody, Internal

Anti-DAZL antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89448
Anti-DAZL antibody, Internal used in Western Blot (WB). GTX89448

Cat. No. GTX89448

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μg ($399)
See all DAZL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89448-PEP).

Predict Reactivity

Human, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-GNGPQKKSVDR, from the internal region of the protein sequence according to NP_001342.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10722773

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

deleted in azoospermia like , DAZH , DAZL1 , DAZLA , SPGYLA

Cellular Localization

Cytoplasm , Nucleus

Background

The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-DAZL antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89448

GTX89448 IHC-P Image

IHC-P analysis of mouse testis using GTX89448 DAZL antibody, Internal.

Anti-DAZL antibody, Internal used in Western Blot (WB). GTX89448

GTX89448 WB Image

WB analysis of rat testes lysate using GTX89448 DAZL antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Le Rolle M et al. Proc Natl Acad Sci U S A 2021; 118 (30) Arrest of WNT/β-catenin signaling enables the transition from pluripotent to differentiated germ cells in mouse ovaries.
Reactivity : Mouse
Chassot AA et al. Sci Adv 2020; 6 (21) : eaaz1261 Retinoic acid synthesis by ALDH1A proteins is dispensable for meiosis initiation in the mouse fetal ovary.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399