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DBC1 antibody [N1N2], N-term

Anti-DBC1 antibody [N1N2], N-term used in Western Blot (WB). GTX118477
Anti-DBC1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118477
Anti-DBC1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118477
Anti-DBC1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118477

Cat. No. GTX118477

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DBC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DBC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885437

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BMP/retinoic acid inducible neural specific 1 , DBC1 , DBCCR1 , FAM5A

Cellular Localization

Cytoplasm

Background

This gene is located within a chromosomal region that shows loss of heterozygosity in some bladder cancers. It contains a 5' CpG island that may be a frequent target of hypermethylation, and it may undergo hypermethylation-based silencing in some bladder cancers. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DBC1 antibody [N1N2], N-term used in Western Blot (WB). GTX118477

GTX118477 WB Image

DBC1 antibody detects DBC1 protein by Western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
7.5 % SDS-PAGE
DBC1 antibody (GTX118477) dilution: 1:500

Anti-DBC1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118477

GTX118477 ICC/IF Image

DBC1 antibody [N1N2], N-term detects DBC1 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 100% MeOH for 5 min.
Green: DBC1 protein stained by DBC1 antibody [N1N2], N-term (GTX118477) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-DBC1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118477

GTX118477 IHC-P Image

DBC1 antibody [N1N2], N-term detects DBC1 protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
DBC1 antibody [N1N2], N-term (GTX118477) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DBC1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX118477

GTX118477 IHC-P Image

DBC1 antibody [N1N2], N-term detects DBC1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
DBC1 antibody [N1N2], N-term (GTX118477) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169