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DBI antibody, N-term

Anti-DBI antibody, N-term used in Western Blot (WB). GTX47770
Anti-DBI antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47770

Cat No. GTX47770

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Positive Control

human fetal liver

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human DBI

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

diazepam binding inhibitor, acyl-CoA binding protein , ACBD1 , ACBP , CCK-RP , EP

Cellular Localization

Endoplasmic reticulum,Golgi apparatus

Background

This gene encodes diazepam binding inhibitor, a protein that is regulated by hormones and is involved in lipid metabolism and the displacement of beta-carbolines and benzodiazepines, which modulate signal transduction at type A gamma-aminobutyric acid receptors located in brain synapses. The protein is conserved from yeast to mammals, with the most highly conserved domain consisting of seven contiguous residues that constitute the hydrophobic binding site for medium- and long-chain acyl-Coenzyme A esters. Diazepam binding inhibitor is also known to mediate the feedback regulation of pancreatic secretion and the postprandial release of cholecystokinin, in addition to its role as a mediator in corticotropin-dependent adrenal steroidogenesis. Three pseudogenes located on chromosomes 6, 8 and 16 have been identified. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DBI antibody, N-term used in Western Blot (WB). GTX47770

GTX47770 WB Image

WB analysis of human fetal liver tissue using GTX47770 DBI antibody at 0.2-1μg/ml.

Anti-DBI antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47770

GTX47770 IHC-P Image

IHC-P analysis of human brain tissue using GTX47770 DBI antibody at 5μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf