GeneTex
United States (US)

DC8 antibody

GTX104452_39904_WB_R_19011506_393.jpg
GTX104452_IFA_19011506_905.jpg
GTX104452_WB_19011506_834.jpg
GTX104452_39904_WB_M_19011506_941.jpg

Cat No. GTX104452

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HepG2 , C8D30 , Rat2

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DC8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C1orf48 antibody, DKFZp566O1646 antibody, MIS14 antibody, NSL1 antibody, kinetochore-associated protein NSL1 homolog antibody, NSL1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody

Cellular Localization

Nucleus , Kinetochore

Background

This gene encodes a protein with two coiled-coil domains that localizes to kinetochores, which are chromosome-associated structures that attach to microtubules and mediate chromosome movements during cell division. The encoded protein is part of a conserved protein complex that includes two chromodomain-containing proteins and a component of the outer plate of the kinetochore. This protein complex is proposed to bridge centromeric heterochromatin with the outer kinetochore structure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104452_39904_WB_R_19011506_393.jpg

GTX104452 WB Image

DC8 antibody detects NSL1 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
DC8 antibody (GTX104452) dilution: 1:1000

GTX104452_IFA_19011506_905.jpg

GTX104452 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DC8(GTX104452) antibody at 1:200 dilution.

GTX104452_WB_19011506_834.jpg

GTX104452 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
12% SDS PAGE
GTX104452 diluted at 1:1000

GTX104452_39904_WB_M_19011506_941.jpg

GTX104452 WB Image

DC8 antibody detects NSL1 protein by Western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
12 % SDS-PAGE
DC8 antibody (GTX104452) dilution: 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169