GeneTex
United States (US)

DCAMKL2 antibody [N2C2], Internal

GTX105963_39940_IFA_19011506_697.jpg
GTX105963_40030_20161110_WB_19011506_967.jpg

Cat No. GTX105963

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCAMKL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CL2 antibody, CLICK-II antibody, CLICK2 antibody, CLIK2 antibody, DCAMKL2 antibody, DCDC3 antibody, DCDC3B antibody, DCK2 antibody, DKFZp761I032 antibody, MGC45428 antibody, DCLK2 antibody, doublecortin and CaM kinase-like 2 antibody, doublecortin-like and CAM kinase-like 2 antibody, serine/threonine-protein kinase DCLK2 antibody, doublecortin domain-containing protein 3B antibody, CaMK-like CREB regulatory kinase 2 antibody, doublecortin-like kinase 2 antibody

Background

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX105963_39940_IFA_19011506_697.jpg

GTX105963 ICC/IF Image

DCAMKL2 antibody [N2C2], Internal detects DCAMKL2 protein at Cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min.
Green: DCAMKL2 protein stained by DCAMKL2 antibody [N2C2], Internal (GTX105963) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX105963_40030_20161110_WB_19011506_967.jpg

GTX105963 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DCAMKL2 antibody [N2C2], Internal (GTX105963) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169