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DCI antibody [N1C3]

Anti-DCI antibody [N1C3] used in Western Blot (WB). GTX104473
Anti-DCI antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104473

Cat. No. GTX104473

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DCI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

A431 , H1299

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950094

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

enoyl-CoA delta isomerase 1 , DCI

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DCI antibody [N1C3] used in Western Blot (WB). GTX104473

GTX104473 WB Image

Sample(30 ug whole cell lysate)
A: 293T
B: H1299
12% SDS PAGE
GTX104473diluted at 1:500

Anti-DCI antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104473

GTX104473 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using DCI(GTX104473) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169