GeneTex
United States (US)

DCI antibody [N1C3]

GTX104473_WB_19011506_224.jpg
GTX104473_39939_IHC_19011506_797.jpg

Cat No. GTX104473

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DCI antibody, "3,2 trans-enoyl-Coenzyme A isomerase antibody", "3,2 trans-enoyl-CoA isomerase antibody", "3,2-trans-enoyl-CoA isomerase, mitochondrial antibody", "dodecenoyl-CoA delta isomerase (3,2 trans-enoyl-CoA isomerase) antibody", acetylene-allene isomerase antibody, "delta3, delta2-enoyl-CoA isomerase antibody", dodecenoyl-CoA isomerase antibody, "enoyl-CoA delta isomerase 1, mitochondrial antibody", "D3,D2-enoyl-CoA isomerase antibody", "delta(3),Delta(2)-enoyl-CoA isomerase antibody", "dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase) antibody"

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104473_WB_19011506_224.jpg

GTX104473 WB Image

Sample(30 ug whole cell lysate)
A: 293T
B: H1299
12% SDS PAGE
GTX104473diluted at 1:500

GTX104473_39939_IHC_19011506_797.jpg

GTX104473 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using DCI(GTX104473) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169