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DCI antibody [N1C3]

Anti-DCI antibody [N1C3] used in Western Blot (WB). GTX104473
Anti-DCI antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104473

Cat No. GTX104473

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enoyl-CoA delta isomerase 1 , DCI

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DCI antibody [N1C3] used in Western Blot (WB). GTX104473

GTX104473 WB Image

Sample(30 ug whole cell lysate)
A: 293T
B: H1299
12% SDS PAGE
GTX104473diluted at 1:500

Anti-DCI antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104473

GTX104473 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using DCI(GTX104473) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for DCI antibody [N1C3] (GTX104473). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DCI antibody [N1C3] (GTX104473). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf