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DCI antibody

Anti-DCI antibody used in Western Blot (WB). GTX53991
Anti-DCI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53991

Cat No. GTX53991

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 63-302 of human ECI1 (NP_001910.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

enoyl-CoA delta isomerase 1 , DCI

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-DCI antibody used in Western Blot (WB). GTX53991

GTX53991 WB Image

WB analysis of various sample lysates using GTX53991 DCI antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-DCI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53991

GTX53991 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX53991 DCI antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf