Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.01 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888285
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
deoxycytidine kinase
Cellular Localization
Nucleus
Background
Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]
DCK antibody [GT10412] detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: DCK protein stained by DCK antibody [GT10412] (GTX632065) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX632065 IP Image
Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 μg of DCK antibody [GT10412] (GTX632065) or DCK antibody (GTX102800). Western blot analysis was performed using DCK antibody [GT10412] (GTX632065) diluted at 1:500. EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.
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GTX632065 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:500.
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GTX632065 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632065 WB Image
DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.
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GTX632065 IHC-P Image
DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632065 IHC-P Image
DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632065 WB Image
DCK antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX632065) diluted at a dilution of 1:500.
Show More +
GTX632065 WB Image
DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.