DCK antibody [GT10412]

Cat No. GTX632065

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	GT10412
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($329), 25 μl ($169)

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , 293T , A549 , H1299 , HCT116 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , PC-12 , Rat2 , DCK-transfected 293T

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888285

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

deoxycytidine kinase

Cellular Localization

Nucleus

Background

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]

Database

Gene ID: 1633 DCK
UniProt: P27707 DCK

Research Area

DATA IMAGES

GTX632065 ICC/IF Image

DCK antibody [GT10412] detects DCK protein at nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: DCK protein stained by DCK antibody [GT10412] (GTX632065) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX632065 IP Image

Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 μg of DCK antibody [GT10412] (GTX632065) or DCK antibody (GTX102800).
Western blot analysis was performed using DCK antibody [GT10412] (GTX632065) diluted at 1:500.
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

GTX632065 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:500.

GTX632065 WB Image

DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.

GTX632065 IHC-P Image

DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
DCK antibody [GT10412] (GTX632065) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632065 IHC-P Image

DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
DCK antibody [GT10412] (GTX632065) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632065 WB Image

DCK antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX632065) diluted at a dilution of 1:500.

GTX632065 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Cho CJ et al. Oncol Lett 2019; 18 (6) : 6869-6876 The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol.

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REVIEW

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Application Reference

Cho CJ et al. Oncol Lett 2019; 18 (6):6869-6876 The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol.

SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 329

25 μl

$ 169

Qty Custom Conjugations

DCK antibody [GT10412]

Cat No. GTX632065

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX632065 ICC/IF Image

GTX632065 IP Image

GTX632065 WB Image

GTX632065 WB Image

GTX632065 IHC-P Image

GTX632065 IHC-P Image

GTX632065 WB Image

GTX632065 WB Image

GTX632065 WB Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Contact

Company

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