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DCK antibody [GT10412]

Overexpression
Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065
Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065
Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065
Anti-DCK antibody [GT10412] used in IHC (Paraffin sections) (IHC-P). GTX632065
Anti-DCK antibody [GT10412] used in IHC (Paraffin sections) (IHC-P). GTX632065
Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065
Anti-DCK antibody [GT10412] used in Immunoprecipitation (IP). GTX632065
Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065
Anti-DCK antibody [GT10412] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632065

Cat. No. GTX632065

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT10412

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DCK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2 , DCK-transfected 293T

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.01 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888285

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

deoxycytidine kinase

Cellular Localization

Nucleus

Background

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065

GTX632065 WB Image

DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.

Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065

GTX632065 WB Image

DCK antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX632065) diluted at a dilution of 1:500.

Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065

GTX632065 WB Image

DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.

Anti-DCK antibody [GT10412] used in IHC (Paraffin sections) (IHC-P). GTX632065

GTX632065 IHC-P Image

DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
DCK antibody [GT10412] (GTX632065) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DCK antibody [GT10412] used in IHC (Paraffin sections) (IHC-P). GTX632065

GTX632065 IHC-P Image

DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
DCK antibody [GT10412] (GTX632065) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065

GTX632065 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:500.

Anti-DCK antibody [GT10412] used in Immunoprecipitation (IP). GTX632065

GTX632065 IP Image

Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 μg of DCK antibody [GT10412] (GTX632065) or DCK antibody (GTX102800).
Western blot analysis was performed using DCK antibody [GT10412] (GTX632065) diluted at 1:500.
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

Anti-DCK antibody [GT10412] used in Western Blot (WB). GTX632065

GTX632065 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-DCK antibody [GT10412] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632065

GTX632065 ICC/IF Image

DCK antibody [GT10412] detects DCK protein at nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: DCK protein stained by DCK antibody [GT10412] (GTX632065) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Cho CJ et al. Oncol Lett 2019; 18 (6) : 6869-6876 The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations