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DCK antibody

Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800
Anti-DCK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800
Anti-DCK antibody used in Immunoprecipitation (IP). GTX102800
Anti-DCK antibody used in Western Blot (WB). GTX102800

Cat No. GTX102800

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A549 , H1299 , PC-12 , Rat2 , DCK-transfected 293T

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

deoxycytidine kinase

Cellular Localization

Nucleus

Background

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]

Database

DATA IMAGES
Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800

GTX102800 IHC-P Image

DCK antibody detects DCK protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DCK antibody (GTX102800) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

DCK antibody detects DCK protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12% SDS-PAGE
DCK antibody (GTX102800) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in IHC (Paraffin sections) (IHC-P). GTX102800

GTX102800 IHC-P Image

DCK antibody detects DCK protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
DCK stained by DCK antibody (GTX102800) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DCK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102800

GTX102800 ICC/IF Image

DCK antibody detects DCK protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DCK protein stained by DCK antibody (GTX102800) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

DCK antibody detects DCK protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCK antibody used in Immunoprecipitation (IP). GTX102800

GTX102800 IP Image

Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 μg of DCK antibody (GTX102800).
Western blot analysis was performed using DCK antibody (GTX102800).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DCK antibody used in Western Blot (WB). GTX102800

GTX102800 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (GTX102800) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169