APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , K562 Cell Lysate(GTX27911)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
DCLK3 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK3.The immunogen is located within the last 50 amino acids of DCLK3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
doublecortin like kinase 3 , CLR , DCAMKL3 , DCDC3C , DCK3
Cellular Localization
Cytoplasm,Nucleus
Background
DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization.
Database
Research Area
DATA IMAGES
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GTX85203 WB Image
WB analysis of K562 cell lysate using GTX85203 DCLK3 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX85203 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85203 DCLK3 antibody.
Working concentration : 5 μg/ml
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GTX85203 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX85203 DCLK3 antibody.
Working concentration : 20 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin
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GTX85203 IHC-P Image
IHC-P analysis of human brain tissue using GTX85203 DCLK3 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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