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DCP1A antibody

Anti-DCP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX04801
Anti-DCP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX04801
Anti-DCP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04801
Anti-DCP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX04801
Anti-DCP1A antibody used in Western Blot (WB). GTX04801

Cat. No. GTX04801

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all DCP1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Bovine, Dog, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DCP1A(Accession Q9NPI6), corresponding to amino acid residues N113-R163.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DCP1A,HSA275986,Nbla00360,SMAD4IP1,SMIF,decapping mRNA 1A

Cellular Localization

Cytoplasm,P-body,Nucleus

Background

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-DCP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX04801

GTX04801 IHC-P Image

IHC-P analysis of rat testis tissue using GTX04801 DCP1A antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-DCP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX04801

GTX04801 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX04801 DCP1A antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-DCP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04801

GTX04801 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04801 DCP1A antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-DCP1A antibody used in IHC (Paraffin sections) (IHC-P). GTX04801

GTX04801 IHC-P Image

IHC-P analysis of rat brain tissue using GTX04801 DCP1A antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-DCP1A antibody used in Western Blot (WB). GTX04801

GTX04801 WB Image

WB analysis of various samples using GTX04801 DCP1A antibody.
Lane 1 : MCF-7 whole cell lysate treated with blocking peptide
Lane 2 : MCF-7 whole cell lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : C6 whole cell lysate

REFERENCE

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REVIEW

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Package List Price ($)
$ 399