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DCP2 antibody

Anti-DCP2 antibody used in Western Blot (WB). GTX118062
Anti-DCP2 antibody used in Western Blot (WB). GTX118062
Anti-DCP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118062
Anti-DCP2 antibody used in Western Blot (WB). GTX118062

Cat. No. GTX118062

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Zebrafish, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all DCP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

IMR32

Predict Reactivity

Rat, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177617

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

decapping mRNA 2 , NUDT20

Cellular Localization

Cytoplasm , P-body , Nucleus

Background

DCP2 is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end (Fenger-Gron et al., 2005 [PubMed 16364915]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DCP2 antibody used in Western Blot (WB). GTX118062

GTX118062 WB Image

DCP2 antibody detects DCP2 protein by Western blot analysis.
A. 30 μg 24hr whole zebrafish lysate/extract
10 % SDS-PAGE
DCP2 antibody (GTX118062) dilution: 1:500

Anti-DCP2 antibody used in Western Blot (WB). GTX118062

GTX118062 WB Image

Sample (30 μg of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX118062 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DCP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX118062

GTX118062 IHC-P Image

DCP2 antibody detects DCP2 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DCP2 antibody (GTX118062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DCP2 antibody used in Western Blot (WB). GTX118062

GTX118062 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DCP2 antibody (GTX118062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ibayashi M et al. Biochem Biophys Res Commun 2021; 555 : 128-133 mRNA decapping factor Dcp1a is essential for embryonic growth in mice.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169