GeneTex
United States (US)

DCTD antibody

GTX108663_41052_WB_B_19011507_325.jpg
GTX108663_41052_WB_19011507_418.jpg
GTX108663_41052_IHC_19011507_585.jpg

Cat No. GTX108663

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

NT2D1 , U87-MG , SK-N-SH , DCTD transfected 293T lysates

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human DCTD Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dcmp Deaminase,Dctd

Background

The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX108663_41052_WB_B_19011507_325.jpg

GTX108663 WB Image

Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: DCTD transfected 293T lysates
12% SDS PAGE
GTX108663 diluted at 1:5000

GTX108663_41052_WB_19011507_418.jpg

GTX108663 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: U87-MG
C: SK-N-SH
12% SDS PAGE
GTX108663 diluted at 1:1000

GTX108663_41052_IHC_19011507_585.jpg

GTX108663 IHC-P Image

DCTD antibody detects DCTD protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
DCTD antibody (GTX108663) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169