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DCTD antibody

Anti-DCTD antibody used in Western Blot (WB). GTX127971
Anti-DCTD antibody used in IHC (Paraffin sections) (IHC-P). GTX127971
Anti-DCTD antibody used in Western Blot (WB). GTX127971

Cat No. GTX127971

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH , human DCTD-transfected 293T cells

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCTD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dCMP deaminase

Background

The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DCTD antibody used in Western Blot (WB). GTX127971

GTX127971 WB Image

DCTD antibody detects DCTD protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human DCTD-transfected 293T cells
12% SDS PAGE
DCTD antibody (GTX127971) dilution: 1:5000

Anti-DCTD antibody used in IHC (Paraffin sections) (IHC-P). GTX127971

GTX127971 IHC-P Image

DCTD antibody detects DCTD protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
DCTD antibody (GTX127971) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DCTD antibody used in Western Blot (WB). GTX127971

GTX127971 WB Image

DCTD antibody detects DCTD protein by Western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
B. 30 μg PC-3 whole cell lysate/extract
C. 30 μg U87-MG whole cell lysate/extract
D. 30 μg SK-N-SH whole cell lysate/extract
12% SDS PAGE
DCTD antibody (GTX127971) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169