APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 10μg/ml |
| 2-4μg/ml |
| 10μg/ml |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells.
IHC-P
Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX11806-PEP).
Product Note
This antibody is expected to recognise all reported isoforms.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
dynactin subunit 1 , DAP-150 , DP-150 , P135
Cellular Localization
Cytoplasm , Cytoplasm, cytoskeleton
Background
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
|
GTX11806 WB Image
WB analysis of human cerebellum lysate using GTX11806 DCTN1 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX11806 FACS Image
FACS analysis of PFA fixed HeLa cells using GTX11806 DCTN1 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
|
|
|
GTX11806 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX11806 DCTN1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
|
|
|
GTX11806 IHC-P Image
IHC-P analysis of human cerebellum using GTX11806 DCTN1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml
|
|
|
GTX11806 ICC/IF Image
ICC/IF analysis of PFA fixed HeLa cells using GTX11806 DCTN1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
|
REFERENCE
There are currently no references for DCTN1 antibody, C-term (GTX11806). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DCTN1 antibody, C-term (GTX11806). Be the first to share your experience with this product.
