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DCTN1 antibody, C-term

Anti-DCTN1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11806
Anti-DCTN1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX11806
Anti-DCTN1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11806
Anti-DCTN1 antibody, C-term used in Flow cytometry (FACS). GTX11806
Anti-DCTN1 antibody, C-term used in Western Blot (WB). GTX11806

Cat No. GTX11806

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2μg/ml
ICC/IF 10μg/ml
IHC-P 2-4μg/ml
FACS 10μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells.

IHC-P
Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells.

Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX11806-PEP).

Product Note

This antibody is expected to recognise all reported isoforms.

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dynactin subunit 1 , DAP-150 , DP-150 , P135

Cellular Localization

Cytoplasm , Cytoplasm, cytoskeleton

Background

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-DCTN1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11806

GTX11806 ICC/IF Image

ICC/IF analysis of PFA fixed U2OS cells using GTX11806 DCTN1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-DCTN1 antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX11806

GTX11806 IHC-P Image

IHC-P analysis of human cerebellum using GTX11806 DCTN1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2.5μg/ml

Anti-DCTN1 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX11806

GTX11806 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX11806 DCTN1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-DCTN1 antibody, C-term used in Flow cytometry (FACS). GTX11806

GTX11806 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX11806 DCTN1 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-DCTN1 antibody, C-term used in Western Blot (WB). GTX11806

GTX11806 WB Image

WB analysis of human cerebellum lysate using GTX11806 DCTN1 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339