Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using dynactin 1(GTX100231) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DCTN1 antibody [C3], C-term detects DCTN1 protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DCTN1(GTX100231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.