GeneTex
United States (US)

DCTN1 antibody [C3], C-term

GTX100231_39391_20160106_IHC-P_R_19011506_249.jpg
GTX100231_40436_20171124_WB_2_19011506_442.jpg
GTX100231_40436_20171020_WB_M_R_19011506_352.jpg
GTX100231_IHC_19011506_695.jpg
GTX100231_40436_20171124_WB_19011506_619.jpg
GTX100231_40436_IFA_19011506_762.jpg
GTX100231_40436_20181228_WB_M_tissue_19011519_892.jpg

Cat No. GTX100231

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , Jurkat , Raji , NCI-H929 , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DCTN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dynactin Subunit 1,Dap-150,Dp-150,P135,Dctn1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100231_39391_20160106_IHC-P_R_19011506_249.jpg

GTX100231 IHC-P Image

DCTN1 antibody [C3], C-term detects DCTN1 protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100231_40436_20171124_WB_2_19011506_442.jpg

GTX100231 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100231_40436_20171020_WB_M_R_19011506_352.jpg

GTX100231 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100231_IHC_19011506_695.jpg

GTX100231 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using dynactin 1(GTX100231) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100231_40436_20171124_WB_19011506_619.jpg

GTX100231 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100231_40436_IFA_19011506_762.jpg

GTX100231 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DCTN1(GTX100231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX100231_40436_20181228_WB_M_tissue_19011519_892.jpg

GTX100231 WB Image

Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term (GTX100231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169