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DCTN1 antibody

Anti-DCTN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54021
Anti-DCTN1 antibody used in Western Blot (WB). GTX54021
Anti-DCTN1 antibody used in Immunoprecipitation (IP). GTX54021

Cat No. GTX54021

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 945-1139 of human DCTN1 (NP_001128513.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

dynactin subunit 1 , DAP-150 , DP-150 , P135

Cellular Localization

Cytoplasm,Cytoplasm, cytoskeleton

Background

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]

Database

Research Area

DATA IMAGES

Anti-DCTN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54021

GTX54021 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54021 DCTN1 antibody.
Blue : DAPI

Anti-DCTN1 antibody used in Western Blot (WB). GTX54021

GTX54021 WB Image

WB analysis of various sample lysates using GTX54021 DCTN1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DCTN1 antibody used in Immunoprecipitation (IP). GTX54021

GTX54021 IP Image

IP analysis of MCF-7 cell lysate using GTX54021 DCTN1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339