GeneTex
United States (US)

DCTN2 antibody

GTX115435_40464_IFA_18121410_534.jpg
GTX115435_40464_IHC_18121410_768.jpg
GTX115435_40464_WB_18121410_690.jpg

Cat No. GTX115435

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCTN2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dynactin Subunit 2,Dctn50,Dynamitin,Hel-S-77,Rbp50,Dctn2

Cellular Localization

Cytoplasm , cytoskeleton , Membrane

Background

This gene encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115435_40464_IFA_18121410_534.jpg

GTX115435 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using Dynactin 2(GTX115435) antibody at 1:500 dilution.

GTX115435_40464_IHC_18121410_768.jpg

GTX115435 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Dynactin 2(GTX115435) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115435_40464_WB_18121410_690.jpg

GTX115435 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX115435 diluted at 1:3000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169