DCUN1D1 antibody [N1C3]

Cat. No. GTX116559

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all DCUN1D1 products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCUN1D1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618992

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

defective in cullin neddylation 1 domain containing 1 , DCNL1 , DCUN1L1 , RP42 , SCCRO , SCRO , Tes3

Background

Part of an E3 ubiquitin ligase complex for neddylation. Required for neddylation of cullin components of E3 cullin-RING ubiquitin ligase complexes by enhancing the rate of cullins neddylation. Functions to recruit the NEDD8-charged E2 enzyme to the cullin component. Involved in the release of inhibitory effets of CAND1 on cullin-RING ligase E3 complex assembly and activity. Acts also as an oncogene facilitating malignant transformation and carcinogenic progression.

Database

Gene ID: 54165 DCUN1D1
UniProt: Q96GG9 DCUN1D1

Research Area

DATA IMAGES

GTX116559 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: JurKat
C: Raji
12% SDS PAGE
GTX116559 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty